Composite

Part:BBa_K395999:Design

Designed by: Mitsuhiko Odera   Group: iGEM10_Tokyo_Tech   (2010-10-26)


Wolfcoli system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1765
    Illegal NheI site found at 1788
    Illegal NheI site found at 18957
    Illegal NotI site found at 18858
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
    Illegal BglII site found at 4076
    Illegal BglII site found at 17135
    Illegal BglII site found at 17387
    Illegal BamHI site found at 13359
    Illegal BamHI site found at 18827
    Illegal XhoI site found at 2183
    Illegal XhoI site found at 18867
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
    Illegal NgoMIV site found at 9213
    Illegal NgoMIV site found at 12895
    Illegal NgoMIV site found at 13025
    Illegal AgeI site found at 9410
    Illegal AgeI site found at 12110
    Illegal AgeI site found at 17397
    Illegal AgeI site found at 18611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 10633
    Illegal BsaI.rc site found at 17218
    Illegal SapI site found at 17045
    Illegal SapI site found at 17057


Design Notes

test


Source

test

References